Détection des pathogènes par PCR

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la PCR temps réel comme technique alternative aux méthodes de culture traditionnelles sur boîtes de Pétri

Ces méthodes de Biologie Moléculaire mettent en œuvre des étapes d’amplification et de détection d’un fragment d’ADN spécifique au micro-organisme recherché. Elles permettent une meilleure détection du pathogène alimentaire ciblé, et évite des résultats faux-positifs.

Le principe de la PCR est basé sur les étapes suivantes :

Dénaturation par chauffage de la molécule double brin d’ADN
Accroche des primers (amorces) qui sont complémentaires d’une séquence d’ADN
Extension par la DNA polymerase (enzyme)

Cette méthode est reproductible, fiable et permet une libération de lot rapide et sûre entre 24h et 48h.

Le système BAX 5 d’HYGIENA

Le système BAX X5 est un appareil de PCR vous permettant de détecter les pathogènes alimentaires de vos échantillons en se basant sur une méthode d’analyse de l’ADN.

Caractéristiques & avantages

Des résultats non équivoques oui-non en seulement 30 heures pour des échantillons d’aliments et environnementaux
Les primers spécifiquement conçus pour se concentrer sur des séquences génétiques spécifiques qui n’existent que dans les organismes cibles
Système simple à installer et à utiliser.
Méthode BAX System X5 validée AFNOR
Possibilité d’analyser différentes souches avec un total de 32 analyses au même temps.
Kits réactifs prêts à l’emploi

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BAX System X5

Les kits de détection sont disponibles pour les pathogènes suivants (principaux enjeux majeurs dans l’industrie agroalimentaire) :

Salmonella
coli O157 :H7
Genus Listeria
monocytogenes

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Test PCR L. monocytogenes

D’autres matrices sont disponibles avec notre système Bax Q7, n’hésitez pas à nous consulter pour avoir davantage d’information !

Salmonella
Coli O157:H7
Shiga toxin-producing E. coli (STEC)
Genus Listeria
Monocytogenes
Cronobacter (E. sakazakii)
Campylobacter jejuni/coli/lari
Shigella
Staphylococcus areus
Vibrio cholerae/ parahaemolyticus/ vulnificusYeast and mold

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la PCR temps réel comme technique alternative aux méthodes de culture traditionnelles sur boîtes de Pétri

Le système BAX 5 d’HYGIENA

Caractéristiques & avantages

BAX System X5

Test PCR E. coli O157:H7

Test PCR Salmonelle

Test PCR Listeria

Test PCR L. monocytogenes

Paramètres de confidentialité

Nos produits